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n gruberi ckk  (Addgene inc)


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    Addgene inc n gruberi ckk
    N Gruberi Ckk, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n gruberi ckk/product/Addgene inc
    Average 88 stars, based on 1 article reviews
    n gruberi ckk - by Bioz Stars, 2026-04
    88/100 stars

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    ( A ) Left: Coronal section of injection site into VTA and starter cells location for Cre-dependent monosynaptic retrograde-tracing experiments. Right: FISH for RbV-N to demonstrate the location of rabies infected cells in the VTA. ( B ) Negative control for EnvA pseudotyping of the rabies virus (EnvA-RbV-GFP) showing a coronal section following injection of EnvA-RbV-GFP into the VTA without prior infection by AAV-TVA-mCh. Without co injecting AAV-DIO-TVA-mCh, EnvA-RbV-GFP cannot infect neurons, thus no GFP expression. ( C ) Controls for specificity of starter cell populations. EnvA-RbV-GFP was injected into the VTA of VGAT-IRES-Cre (left) and DAT-IRES-Cre (right) mice following injection of AAV-DIO-TVA-mCh, but not AAV-DIO-RVG. Therefore, rabies virus could not spread from initially infected neurons. Left: Quantification of fluorescence coverage of RbV-N+ cells in VGAT-IRES-Cre mice, almost all (99%) infected cells expressed detectable levels of Cre demonstrating specificity of the AAV-DIO-TVA virus (n = 319 cells, two mice). Right: Quantification of fluorescence coverage of RbV-N+ cells in DAT-IRES-Cre mice, almost all infected cells (95%) expressed detectable levels of Cre demonstrating specificity of the AAV-DIO-TVA virus (n = 522 cells, two mice). ( D ) Sample VTA FISH image of starter cell location for monosynaptic retrograde tracing experiments performed in VGAT-IRES-Cre mice. Inset depicts two putative starter cells (arrowheads) that express RbV-N (green) and Cre (red). VTA is outlined by white dashed line. ( E ) Sample VTA FISH image of starter cell location for monosynaptic retrograde tracing experiments performed in DAT-IRES-Cre mice. Inset depicts three putative starter cells (arrowheads) that express RbV-N (green) and Cre (red). VTA is outlined by white dashed line. ( F ) Left: Quantification of fluorescence coverage of single putative starter cells ( Cre+ and RbV-N+ ) for FISH of Cre and Slc17a6 in VTA neurons of the VGAT-IRES-Cre animals (n = 567 cells, four mice). Right: The proportion of putative starter cells that expressed Slc17a6 . There is a subset of VGAT-IRES-Cre+ neurons in the VTA that co-express Slc17a6 . ( G ) Left: Quantification of fluorescence coverage of single putative starter cells ( <t>Slc6a3+</t> and RbV-N+ ) for FISH of Slc6a3 and Slc32a1 in VTA neurons of the DAT-IRES-Cre animals (n = 566 cells, three mice). Right: The proportion of putative starter cells that expressed Slc32a1 . Note: Approximately 12% (451/3754 cells/4 mice) of Slc32a1 + VTA neurons were starter cells in VGAT-IRES-Cre retrograde labeling experiments. In DAT-IRES-Cre experiments, approximately 18% (468/2598 cells/3 mice) of Slc6a3 + VTA neurons were starter cells. Filled rectangles represent the mean and error bars are ± SEM, Abbreviations: IPN - interpeduncular nucleus, SNr – substantia nigra reticulata.
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    ( A ) Left: Coronal section of injection site into VTA and starter cells location for Cre-dependent monosynaptic retrograde-tracing experiments. Right: FISH for RbV-N to demonstrate the location of rabies infected cells in the VTA. ( B ) Negative control for EnvA pseudotyping of the rabies virus (EnvA-RbV-GFP) showing a coronal section following injection of EnvA-RbV-GFP into the VTA without prior infection by AAV-TVA-mCh. Without co injecting AAV-DIO-TVA-mCh, EnvA-RbV-GFP cannot infect neurons, thus no GFP expression. ( C ) Controls for specificity of starter cell populations. EnvA-RbV-GFP was injected into the VTA of VGAT-IRES-Cre (left) and DAT-IRES-Cre (right) mice following injection of AAV-DIO-TVA-mCh, but not AAV-DIO-RVG. Therefore, rabies virus could not spread from initially infected neurons. Left: Quantification of fluorescence coverage of RbV-N+ cells in VGAT-IRES-Cre mice, almost all (99%) infected cells expressed detectable levels of Cre demonstrating specificity of the AAV-DIO-TVA virus (n = 319 cells, two mice). Right: Quantification of fluorescence coverage of RbV-N+ cells in DAT-IRES-Cre mice, almost all infected cells (95%) expressed detectable levels of Cre demonstrating specificity of the AAV-DIO-TVA virus (n = 522 cells, two mice). ( D ) Sample VTA FISH image of starter cell location for monosynaptic retrograde tracing experiments performed in VGAT-IRES-Cre mice. Inset depicts two putative starter cells (arrowheads) that express RbV-N (green) and Cre (red). VTA is outlined by white dashed line. ( E ) Sample VTA FISH image of starter cell location for monosynaptic retrograde tracing experiments performed in DAT-IRES-Cre mice. Inset depicts three putative starter cells (arrowheads) that express RbV-N (green) and Cre (red). VTA is outlined by white dashed line. ( F ) Left: Quantification of fluorescence coverage of single putative starter cells ( Cre+ and RbV-N+ ) for FISH of Cre and Slc17a6 in VTA neurons of the VGAT-IRES-Cre animals (n = 567 cells, four mice). Right: The proportion of putative starter cells that expressed Slc17a6 . There is a subset of VGAT-IRES-Cre+ neurons in the VTA that co-express Slc17a6 . ( G ) Left: Quantification of fluorescence coverage of single putative starter cells ( <t>Slc6a3+</t> and RbV-N+ ) for FISH of Slc6a3 and Slc32a1 in VTA neurons of the DAT-IRES-Cre animals (n = 566 cells, three mice). Right: The proportion of putative starter cells that expressed Slc32a1 . Note: Approximately 12% (451/3754 cells/4 mice) of Slc32a1 + VTA neurons were starter cells in VGAT-IRES-Cre retrograde labeling experiments. In DAT-IRES-Cre experiments, approximately 18% (468/2598 cells/3 mice) of Slc6a3 + VTA neurons were starter cells. Filled rectangles represent the mean and error bars are ± SEM, Abbreviations: IPN - interpeduncular nucleus, SNr – substantia nigra reticulata.
    N Gruberi Ckk, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n gruberi ckk/product/Addgene inc
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    n gruberi ckk - by Bioz Stars, 2026-04
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    ( A ) Left: Coronal section of injection site into VTA and starter cells location for Cre-dependent monosynaptic retrograde-tracing experiments. Right: FISH for RbV-N to demonstrate the location of rabies infected cells in the VTA. ( B ) Negative control for EnvA pseudotyping of the rabies virus (EnvA-RbV-GFP) showing a coronal section following injection of EnvA-RbV-GFP into the VTA without prior infection by AAV-TVA-mCh. Without co injecting AAV-DIO-TVA-mCh, EnvA-RbV-GFP cannot infect neurons, thus no GFP expression. ( C ) Controls for specificity of starter cell populations. EnvA-RbV-GFP was injected into the VTA of VGAT-IRES-Cre (left) and DAT-IRES-Cre (right) mice following injection of AAV-DIO-TVA-mCh, but not AAV-DIO-RVG. Therefore, rabies virus could not spread from initially infected neurons. Left: Quantification of fluorescence coverage of RbV-N+ cells in VGAT-IRES-Cre mice, almost all (99%) infected cells expressed detectable levels of Cre demonstrating specificity of the AAV-DIO-TVA virus (n = 319 cells, two mice). Right: Quantification of fluorescence coverage of RbV-N+ cells in DAT-IRES-Cre mice, almost all infected cells (95%) expressed detectable levels of Cre demonstrating specificity of the AAV-DIO-TVA virus (n = 522 cells, two mice). ( D ) Sample VTA FISH image of starter cell location for monosynaptic retrograde tracing experiments performed in VGAT-IRES-Cre mice. Inset depicts two putative starter cells (arrowheads) that express RbV-N (green) and Cre (red). VTA is outlined by white dashed line. ( E ) Sample VTA FISH image of starter cell location for monosynaptic retrograde tracing experiments performed in DAT-IRES-Cre mice. Inset depicts three putative starter cells (arrowheads) that express RbV-N (green) and Cre (red). VTA is outlined by white dashed line. ( F ) Left: Quantification of fluorescence coverage of single putative starter cells ( Cre+ and RbV-N+ ) for FISH of Cre and Slc17a6 in VTA neurons of the VGAT-IRES-Cre animals (n = 567 cells, four mice). Right: The proportion of putative starter cells that expressed Slc17a6 . There is a subset of VGAT-IRES-Cre+ neurons in the VTA that co-express Slc17a6 . ( G ) Left: Quantification of fluorescence coverage of single putative starter cells ( <t>Slc6a3+</t> and RbV-N+ ) for FISH of Slc6a3 and Slc32a1 in VTA neurons of the DAT-IRES-Cre animals (n = 566 cells, three mice). Right: The proportion of putative starter cells that expressed Slc32a1 . Note: Approximately 12% (451/3754 cells/4 mice) of Slc32a1 + VTA neurons were starter cells in VGAT-IRES-Cre retrograde labeling experiments. In DAT-IRES-Cre experiments, approximately 18% (468/2598 cells/3 mice) of Slc6a3 + VTA neurons were starter cells. Filled rectangles represent the mean and error bars are ± SEM, Abbreviations: IPN - interpeduncular nucleus, SNr – substantia nigra reticulata.
    Rbfox1 N, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rbfox1 n/product/Addgene inc
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    ( A ) Left: Coronal section of injection site into VTA and starter cells location for Cre-dependent monosynaptic retrograde-tracing experiments. Right: FISH for RbV-N to demonstrate the location of rabies infected cells in the VTA. ( B ) Negative control for EnvA pseudotyping of the rabies virus (EnvA-RbV-GFP) showing a coronal section following injection of EnvA-RbV-GFP into the VTA without prior infection by AAV-TVA-mCh. Without co injecting AAV-DIO-TVA-mCh, EnvA-RbV-GFP cannot infect neurons, thus no GFP expression. ( C ) Controls for specificity of starter cell populations. EnvA-RbV-GFP was injected into the VTA of VGAT-IRES-Cre (left) and DAT-IRES-Cre (right) mice following injection of AAV-DIO-TVA-mCh, but not AAV-DIO-RVG. Therefore, rabies virus could not spread from initially infected neurons. Left: Quantification of fluorescence coverage of RbV-N+ cells in VGAT-IRES-Cre mice, almost all (99%) infected cells expressed detectable levels of Cre demonstrating specificity of the AAV-DIO-TVA virus (n = 319 cells, two mice). Right: Quantification of fluorescence coverage of RbV-N+ cells in DAT-IRES-Cre mice, almost all infected cells (95%) expressed detectable levels of Cre demonstrating specificity of the AAV-DIO-TVA virus (n = 522 cells, two mice). ( D ) Sample VTA FISH image of starter cell location for monosynaptic retrograde tracing experiments performed in VGAT-IRES-Cre mice. Inset depicts two putative starter cells (arrowheads) that express RbV-N (green) and Cre (red). VTA is outlined by white dashed line. ( E ) Sample VTA FISH image of starter cell location for monosynaptic retrograde tracing experiments performed in DAT-IRES-Cre mice. Inset depicts three putative starter cells (arrowheads) that express RbV-N (green) and Cre (red). VTA is outlined by white dashed line. ( F ) Left: Quantification of fluorescence coverage of single putative starter cells ( Cre+ and RbV-N+ ) for FISH of Cre and Slc17a6 in VTA neurons of the VGAT-IRES-Cre animals (n = 567 cells, four mice). Right: The proportion of putative starter cells that expressed Slc17a6 . There is a subset of VGAT-IRES-Cre+ neurons in the VTA that co-express Slc17a6 . ( G ) Left: Quantification of fluorescence coverage of single putative starter cells ( Slc6a3+ and RbV-N+ ) for FISH of Slc6a3 and Slc32a1 in VTA neurons of the DAT-IRES-Cre animals (n = 566 cells, three mice). Right: The proportion of putative starter cells that expressed Slc32a1 . Note: Approximately 12% (451/3754 cells/4 mice) of Slc32a1 + VTA neurons were starter cells in VGAT-IRES-Cre retrograde labeling experiments. In DAT-IRES-Cre experiments, approximately 18% (468/2598 cells/3 mice) of Slc6a3 + VTA neurons were starter cells. Filled rectangles represent the mean and error bars are ± SEM, Abbreviations: IPN - interpeduncular nucleus, SNr – substantia nigra reticulata.

    Journal: eLife

    Article Title: Anatomical and single-cell transcriptional profiling of the murine habenular complex

    doi: 10.7554/eLife.51271

    Figure Lengend Snippet: ( A ) Left: Coronal section of injection site into VTA and starter cells location for Cre-dependent monosynaptic retrograde-tracing experiments. Right: FISH for RbV-N to demonstrate the location of rabies infected cells in the VTA. ( B ) Negative control for EnvA pseudotyping of the rabies virus (EnvA-RbV-GFP) showing a coronal section following injection of EnvA-RbV-GFP into the VTA without prior infection by AAV-TVA-mCh. Without co injecting AAV-DIO-TVA-mCh, EnvA-RbV-GFP cannot infect neurons, thus no GFP expression. ( C ) Controls for specificity of starter cell populations. EnvA-RbV-GFP was injected into the VTA of VGAT-IRES-Cre (left) and DAT-IRES-Cre (right) mice following injection of AAV-DIO-TVA-mCh, but not AAV-DIO-RVG. Therefore, rabies virus could not spread from initially infected neurons. Left: Quantification of fluorescence coverage of RbV-N+ cells in VGAT-IRES-Cre mice, almost all (99%) infected cells expressed detectable levels of Cre demonstrating specificity of the AAV-DIO-TVA virus (n = 319 cells, two mice). Right: Quantification of fluorescence coverage of RbV-N+ cells in DAT-IRES-Cre mice, almost all infected cells (95%) expressed detectable levels of Cre demonstrating specificity of the AAV-DIO-TVA virus (n = 522 cells, two mice). ( D ) Sample VTA FISH image of starter cell location for monosynaptic retrograde tracing experiments performed in VGAT-IRES-Cre mice. Inset depicts two putative starter cells (arrowheads) that express RbV-N (green) and Cre (red). VTA is outlined by white dashed line. ( E ) Sample VTA FISH image of starter cell location for monosynaptic retrograde tracing experiments performed in DAT-IRES-Cre mice. Inset depicts three putative starter cells (arrowheads) that express RbV-N (green) and Cre (red). VTA is outlined by white dashed line. ( F ) Left: Quantification of fluorescence coverage of single putative starter cells ( Cre+ and RbV-N+ ) for FISH of Cre and Slc17a6 in VTA neurons of the VGAT-IRES-Cre animals (n = 567 cells, four mice). Right: The proportion of putative starter cells that expressed Slc17a6 . There is a subset of VGAT-IRES-Cre+ neurons in the VTA that co-express Slc17a6 . ( G ) Left: Quantification of fluorescence coverage of single putative starter cells ( Slc6a3+ and RbV-N+ ) for FISH of Slc6a3 and Slc32a1 in VTA neurons of the DAT-IRES-Cre animals (n = 566 cells, three mice). Right: The proportion of putative starter cells that expressed Slc32a1 . Note: Approximately 12% (451/3754 cells/4 mice) of Slc32a1 + VTA neurons were starter cells in VGAT-IRES-Cre retrograde labeling experiments. In DAT-IRES-Cre experiments, approximately 18% (468/2598 cells/3 mice) of Slc6a3 + VTA neurons were starter cells. Filled rectangles represent the mean and error bars are ± SEM, Abbreviations: IPN - interpeduncular nucleus, SNr – substantia nigra reticulata.

    Article Snippet: Antisense probes for RbV-N, Gpr151, Sst, Plch1, Pbx3, Rbfox1, Chrm3, Vgf, Cre, Slc17a6, Slc32a1, and Slc6a3 were purchased from Advanced Cell Diagonstics (ACD, http://acdbio.com/ ).

    Techniques: Injection, Retrograde Tracing, Infection, Negative Control, Virus, Expressing, Fluorescence, Labeling

    Journal: eLife

    Article Title: Anatomical and single-cell transcriptional profiling of the murine habenular complex

    doi: 10.7554/eLife.51271

    Figure Lengend Snippet:

    Article Snippet: Antisense probes for RbV-N, Gpr151, Sst, Plch1, Pbx3, Rbfox1, Chrm3, Vgf, Cre, Slc17a6, Slc32a1, and Slc6a3 were purchased from Advanced Cell Diagonstics (ACD, http://acdbio.com/ ).

    Techniques: Virus, Generated, RNAscope, Multiplex Assay, Detection Assay, Software